We next compared an animal's preference for sucrose vs sucralose plus optogenetic activation of MCH neurons (see 'Materials and methods' for the rationale of concentrations chosen).
Mean values ± SD for percent normal [p < 0.0001, Fisher's Exact test comparing the distribution of normal and abnormal animals for each rescue to ced-3 lf);ain-1(lf) ced-3 lfrescue (see 'Main-1 lf ain-1 lfods' for statistical rationale)].
Mean values ± SD for percent normal [p < 0.0001, *compared to mock RNAi, Fisher's Exact test comparing the distributions of normal and abnormal animals (see 'Materials and methods' for statistical rationale)].
(A – C ) Mice with ablated MCH neurons (red filled bars) and their respective controls (blue filled bars) were given the choice of (A ) 0.4 M sucrose vs 1.5 mM sucralose (*p<0.03, ¥p<0.011, see 'Materials and methods' for the rationale of concentrations).
Significance of phenotypes when wt and ain-1 (lf ) animals were fed the indicated RNAi was determined [**p < 0.001, relative to both ain-1 (lf ) fed mock RNAi and to wt fed the given RNAi, Fisher's exact test comparing the distributions of normal and abnormal animals (see 'Materials and methods' for statistical rationale)].
We considered a shift in pI between human and mouse to be high if it was greater than 0.92, and an identical value between human and cow (Additional File 1; see Methods for the rationale behind the choice of these cut-offs).
M. oryzae grown in complete medium was used as the reference condition for each hybridization experiment (see Methods for details and rationale).
To describe the methods and rationale for the first randomized controlled trial conducted by the Urinary Incontinence Treatment Network.
That last one should be of interest to BitTorrent users: here, ISPs will have to make available their methods and rationale for managing their network.
Appropriate methods and instruments are already available to this end, and in this chapter we describe both the methods and rationale for comparison of engineered/regenerating bladders with native bladder.
