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The methods for protein extraction, followed by western blot analysis, were based on protocols from previous studies22,48.
The FoldMiner web server (http://foldminer.stanford.edu/) provides remote access to methods for protein structure alignment and unsupervised motif discovery.
Structural biology is increasingly reliant on elevated throughput methods for protein production.
Affinity chromatography is one of the most popular methods for protein purification.
This understanding can be used to design more powerful prediction methods for protein structural class.
The development of fast and reliable methods for protein determination are of great relevance to a diversity of areas from industry to diagnostics.
This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.
Several methods for protein engineering and evolution make use of cell-free expression, and it is being used to translate our genomic knowledge into functional proteins.
There have been significant recent developments both in our understanding of the molecular mechanisms that control cell cycle progression and in methods for protein structure determination at atomic resolution.
In this chapter we will introduce the commonly used methods for protein modeling and structure prediction, with a particular emphasis on the comparative or homology modeling approaches that are widely used in drug discovery and design.
Fig. 16 Illustrations of genetic recombination methods for protein evolution.
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