Furthermore, development of an effective sterilization method for living P. boydii in the residue increased the applicability of our bioreactor system for practical use in dioxin-contaminated sites.
Strategies for improving specificity, dynamic range or sensitivity, reversibility and integrity of the reporter as well as basic methods for live-cell time-lapse imaging using these reporters are summarized.
Focusing on two relevant analytical methods for live-cell monitoring, we discuss the current challenges of analyzing living 3D samples: microscopy, which is the most widely used technology to observe and examine cell cultures, has been successfully adapted for 3D samples by recording of so-called "z-stacks".
In this chapter, we discuss methods for the expression and imaging of fluorescent proteins in the C. elegans germline, including the design of transgenes for optimal expression, the generation of transgenic worm lines by ballistic bombardment, the construction of multimarker lines by mating, and methods for live imaging of the gonad and early embryo.
Our method has two main advantages over the widely used ordinal-category methods for live mice.
Detailed methods for live cell imaging and McTN scoring were previously described [ 12].
Other very recent and potentially useful technological approaches to go beyond average measurements and explore noise and cell-to-cell variations include super-resolution microscopy methods for live cell imaging, which promise to display single molecule dynamics with second- or even sub-second-scale time resolution.
Differences in category-related brain activity have been demonstrated with various neuroimaging methods in healthy subjects, for living things versus manmade objects, and for several specific object categories such as faces, body parts, animals, fruits/vegetables, buildings, tools and furniture (for a recent review see [7] [9]).
"A master sets goals not for the sake of obtainment, but for a method of living" is a Tai Chi maxim.
