Sentence examples for methods for live from inspiring English sources
In this chapter, we discuss methods for the expression and imaging of fluorescent proteins in the C. elegans germline, including the design of transgenes for optimal expression, the generation of transgenic worm lines by ballistic bombardment, the construction of multimarker lines by mating, and methods for live imaging of the gonad and early embryo.
Our method has two main advantages over the widely used ordinal-category methods for live mice.
Detailed methods for live cell imaging and McTN scoring were previously described [ 12].
Other very recent and potentially useful technological approaches to go beyond average measurements and explore noise and cell-to-cell variations include super-resolution microscopy methods for live cell imaging, which promise to display single molecule dynamics with second- or even sub-second-scale time resolution.
Strategies for improving specificity, dynamic range or sensitivity, reversibility and integrity of the reporter as well as basic methods for live-cell time-lapse imaging using these reporters are summarized.
Focusing on two relevant analytical methods for live-cell monitoring, we discuss the current challenges of analyzing living 3D samples: microscopy, which is the most widely used technology to observe and examine cell cultures, has been successfully adapted for 3D samples by recording of so-called "z-stacks".
Others believe big data can find out the very best ways to achieve better methods for living far longer.
Immunolabeling of intracellular epitopes requires membrane permeabilization, and considering that the pore-forming methods for living cells appear to induce significant structural changes (Humbel et al. 1998), immunolabeling methods for living cells seem to be largely restricted to labeling of proteins with extracellular epitopes.
The feasibility of this method for live cell measurements is demonstrated studying the phagocytosis of protein-coated fluorospheres by fibroblasts.
Using a recombinant bacteriophage, here we report the development of a rapid and sensitive method for live E. coli O157 H7 detection.
We recently established a non-invasive method for live animal imaging to detect phosphorylated γH2AX in response to DNA damage.
