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There are a number of methods for labeling RNA in live cells; we'll introduce the myriad of methods and then focus on one approach for labeling viral RNA, using multiply-labeled tetravalent RNA imaging probes (MTRIPs), which do not require engineering of the target RNAs.
Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.
The objective of this study was to evaluate four different methods for labeling MR1-1 scFv that had been previously investigated for the radioiodinating of an intact anti-epidermal growth factor receptor variant III (anti-EGFRvIII) monoclonal antibody (mAb) L8A4.
The goal of this work was to develop methods for labeling polymeric nanoparticles with an F-18-biotin derivative to enable imaging of their delivery to intact tissue with PET.
Thirdly, there are several methods for labeling one biotin at one terminus of the DNA fragment such as PCR method with one of the two primers biotinylated (this paper), end-labeling using terminal transferase, ligation reaction with a biotinylated adaptor [ 58] or direct generated by company service.
Detailed methods for labeling, hybridizations to the arrays and scanning are described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual, Revision 3, from Affymetrix, and are available upon request.
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We have simultaneously developed a straightforward method for labeling miRNAs in a sample.
A method for labeling cells for positron emission tomography (PET) studies.
We report a highly specific, robust and rapid new method for labeling cell surface proteins with biophysical probes.
Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster.
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits.
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