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Due to low sensitivity in the PCR reactions modified methods for extraction of DNA were tested and in order to concentrate extracts, DNA was eluted in 100 μl of AE buffer supplied by the manufacturer in the final steps of the elution process, which was half of the volume of elution buffer suggested in the manual.
Two principal methods for extraction are considered, namely, ISO-based measurements and the traditional tailoring approach.
But, as the 21st century kicked into gear, stocks of easily extracted fossil fuels began to run low, and industries resorted to increasingly dangerous – and expensive – methods for extraction, often with deadly, environmentally destructive results.
Existing methods for extraction and processing of large fragments of bacterial genomic DNA are manual, time-consuming, and prone to variability in DNA quality and recovery.
The focus of the overview is on empirical methods for extraction of metals from soils as a surrogate for the metal-, species- and soil-type-dependent bioavailable and bioaccessible metal pools.
Areas covered include the evaluation of scale, topology, and orientation of trabecular networks by three-dimensional MRI both ex vivo and in vivo, with particular emphasis on the methods for extraction of structural measures and their mechanical implications in the human skeleton in health and disease.
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"Individuals are able to use other methods for extractions, such as the use of food grade oil such as olive oil, since these methods do not pose the same health and safety risks to the person performing the extraction, and the people and homes around them," the statement says.
Paterson, A. H., Brubaker, C. L. & Wendel, J. F. A rapid method for extraction of cotton (Gossypium spp). genomic DNA suitable for RFLP or PCR analysis.
Finally, the practicality of the method for extraction from urine and plasma samples was investigated.
In this research work, a novel method for extraction of ulvan from green algae is proposed and demonstrated successfully.
Based on these, a rapid and efficient method for extraction and analysis BaP in real water sample was established.
More suggestions(15)
methods for retrieval
methods for production
methods for operation
methods for exploitation
methods for extracting
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means for extraction
methods for mining
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methods for calculation
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