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We conducted two phylogenetic analyses with different outgroup gene samplings, providing essentially similar results: an analysis rooted using the MHCII sequence of the amoebozoan Dictyostelium discoideum and another analysis, rooted with sequences of myosin classes V, VII and X (Additional file 3) (see Methods for explanations).
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(See Methods for explanation of the strata).
When the 37 kg leatherback was in TW ≤25°C, thermal admittance (see Materials & Methods for explanation of thermal admittance) was between 10 and 14 W m−2°C−1 (Table 1).
Finally, the serial autocorrelation analysis of the run length deviation from the mean is not significantly different from zero for any run interval (Fig. 3D), indicating no signs for clusters of short or long runs (see also methods for explanation).
Of the 495 descriptors, 48 obtained variable importance in projection (VIP) values larger than one and were used to choose intra-RT cross-terms (see Methods for explanation of VIP and for further details on selection of intra-RT cross-terms).
aSee Materials and methods for explanation of the three HCC-related gene sets.
Most arrays fell into one of four categories (see methods for explanation): CE-AGC, CE+AGC, AGC and UCO.
There was no significant correlation between the propagule densities of any two genera or any pair of operational taxonomic units (OTUs, see methods for explanation).
We sequenced a total of 11,616 ESTs from the 3' end (see Methods for explanation of sequencing rationale), of which 9,738 were of sufficiently high quality and length to process further.
These effects may relate to small differences in melatonin exposure at the time capsules were removed (see Experiment 3 Methods for explanation) or, in the case of body weight, previously described differential regulation of body weight and reproduction [ 31- 33].
To more thoroughly address the possible genomic feature origins of our small RNA data sets, we used prorated counts (see Material and Methods for explanation) because it takes into account multiple alignments and different features associated with each alignment (Tables 2 and 3).
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