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The aim of this study was to assess the impact of applying different methods for duplicate isolate removal on the MRSA rates.
Studies comparing the potential effect of using different methods for duplicate isolate removal are limited, i.e., most existing analyses are based on data from a single facility or compared only a few of the many different options for duplicate isolate removal (4 – 6 ).
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Benchmarking on reference-free metagenome datasets would have resulted in a set of duplicate clusters and an expected duplication rate but would give no indication of the accuracy of each method for duplicate detection.
Blueprinting, the first economical method for duplicating drawings, was invented in 1842 and introduced in the United States in 1876.
Areas for additional research include methods for analyzing duplicate isolates from a single patient, more sophisticated techniques for modeling patient location, accounting robustly for changes in sampling intensity, methods for using quantitative CUSUMs, and the potential need for corrections for interdependence.
When very large numbers of ESTs are needed, serial-subtractive hybridization is an effective, but technically demanding, method for removing duplicate clones [ 22, 23].
To test the SIP method for identifying duplicate citation pairs not found by eTBLAST, 10 000 citation pairs were selected from region D of Figure 1.
Normalized signal ratios of the loop to scaffold/matrix signal from all aCGH probes for each extraction method from duplicate independent experiments were calculated.
The second approach (counting method) took advantage of the finding that a maximum likelihood method for identifying duplicated genes using the number of members in each FRB family for each species performs remarkably similar to a phylogenetic method (Hahn et al. 2007).
Excel 2003 uses a conditional formula-based method for identifying duplicates, while Excel 2007 and 2010 simplify the process.
The BLAST based methods completely failed for duplicated nodes but succeeds for non-duplicated nodes.
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