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Additionally, we performed a sample size calculation to estimate the number of subjects needed to detect (with 80% power at 5% significance) a difference in tNAA concentration of 4 5% (as observed in our study) between patients and controls, using the means and SDs of tNAA concentrations provided by our data, and standard methods for comparisons of means [Armitage et al., 2002].
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We introduce two baseline methods for comparison.
Other methods for comparison may be developed with better robustness on different lighting conditions.
We use five of the state-of-the-art methods for comparison purposes.
The sizes of search area and template keep same to all methods for comparison equity.
It was also prepared by sonication as well as bulk mixing methods for comparison purpose.
Our implementations include some of the previous methods for comparison purposes as well as our proposed approaches.
Beginning with the development of cophenetic correlations methods for comparison of dendrograms have recently been the object of strong interest.
The actual number of communities generated by HierSymNMF2 is usually less than 5000, as discussed in "Methods for comparison" section.
Two mixing models were used in conjunction with GRG non-linear and Evolutionary optimization methods for comparison.
Note however that we will also report the results of dk-NN and KDE methods for comparison reason (methods explained further below).
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