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Tissue engineering approaches are potent methods for cartilage repair.
However, current methods for cartilage morphometry take many hours per knee and require extensive training on the use of the associated software.
The present study relied on fully automated computer-based MRI methods for cartilage status assessment and a standardized biochemical marker measured through standard ELISA techniques.
Building on our methods for cartilage tissue engineering using different cells and biomaterials [6,23], we have explored the factors that are most important in driving an effective integration between tissues.
The current cell based treatment methods for cartilage repair are based on the migration of bone marrow derived stem cells to the site of injury and include arthroscopic abrasion, subchondral drilling, and microfracture [ 2].
Several semi-automatic methods for cartilage quantification have been reported [ 17- 19], including scoring systems integrating several joint features – for example, the Whole-Organ Magnetic Resonance Imaging Score [ 20].
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Therefore, glucosamine modified PEG hydrogels facilitated the chondrogenesis of hBMSCs, which might represent a new method for cartilage repair using a tissue-engineering approach.
Arthroscopy is considered the most valid method for cartilage evaluation.
MRI is currently the standard method for cartilage evaluation as it allows morphological assessment of the cartilage surface, thickness, volume and subchondral bone [ 27– 33].
As cartilage regeneration research matures, long-term follow-up and larger comparative trials will ultimately establish the optimal method for cartilage repair.
Tissue engineering provides a new method for cartilage repair, but it is a complex process involving interactions between the scaffold, the seeded cells and various cytokines.
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