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DNA fingerprinting methods for bacterial identification centre primarily on the use of the polymerase chain reaction (PCR).
Conventional detection methods for bacterial pathogens and toxins are time consuming and laborious, requiring certain sophisticated instruments and trained personnel.
Viable methods for bacterial biofilm remediation require a fundamental understanding of biofilm mechanical properties and their dependence on dynamic environmental conditions.
Rapid and specific detection methods for bacterial agents in drinking water are important for disease prevention and responding to suspected contamination events.
In addition to established methods for bacterial load determinations, new technologies are emerging that allow us to specifically evaluate effects of vaccines on the pathology of the disease process and the expression by the host of cell mediated immunity.
The most common methods for bacterial transformation are heat shock and electroporation, with advantage of high efficiency (up to 108 CFU/μg plasmid).
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We have used nano-sized acicular material to establish a novel method for bacterial transformation.
In total, we present a facile and rapid method for bacterial transformation, which has comparable efficiency with the common method.
In total, the aim of this study was to present a rapid and effective method for bacterial transformation that is an essential step in recombinant DNA technology.
MALDI-TOF mass spectrometry (MS) has been suggested as a fast and reliable method for bacterial identification [10], [11], based on protein profiles characteristic of each microorganism.
Quantitative real-time PCR (qPCR) offers a culture-independent method for bacterial quantification that may improve diagnosis of CF airway infections; however, the reliability of qPCR applied to CF airway specimens is unknown.
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