Transfection was carried out at approximately 50% confluence using the Calcium phosphate method following standard procedures.
DNA was extracted by the proteinase K/phenol-chloroform method, following standard procedures [25].
The relative level of SRY transcripts in blood was calculated by the ΔΔCt method following standard protocol (ABI).
Tumor tissue portions collected in RNA later (Ambion, Austin, TX, USA), were used for DNA isolation by phenol chloroform extraction method, following standard protocol [ 23].
The composition of each product has been determined quantitatively in weight percentages using the following standard methods of analysis: (a) Carbon: Dumas method.
Alizarin red photometric method of fluoride analysis was carried out using the spectrophotometer at 570 nm wavelength following standard methods as described in APHA (2005).
Trace metal analysis was carried out using Atomic Absorption Spectrometer AASS) following standard methods given in APHA (2012).
Estimation of the abundance and habitat characterization was done through field surveys following standard methods.
In addition, the MLSS and MLVSS were determined following standard methods (APHA et al. 2012).
The synthetic characters of the community were computed following standard methods.
