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Our model development methods followed established time series forecasting procedures.
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The staining procedures followed established methods [34].
The HPLC gradient consisted of solvent A [1.5% H3PO4] and solvent B [HOAc CH3CN H3PO4:H2O (20∶24∶1.5∶54.5)], mixed using a linear gradient starting with 80% A, decreasing to 33% A at 30 min, 10% A at 33 min and 0% at 39.3 min. HPLC peak identification and calculations followed established methods [14].
Reconstruction and model development of the metabolic network followed established methods [ 5, 3].
Although we followed established methods of instrument development, taken together our items did not perform well psychometrically [ 15].
Sperm morphometric measures also followed established methods (Gage et al. 1998), with 5 μL of sperm-extender subsamples being preserved before activation in 400 μL of 5% formalin.
In addition, we followed established methods and excluded all controls presenting to the hospital with illnesses other than malaria or pneumonia [ 9], as documented in the hospital register.
This systematic review followed established methods for systematic reviews 11 and builds on a scoping review conducted by our group on the use of social media among patients and caregivers.
The methods used to perform this review involved both electronic and manual components, and followed established best methods used in the science of systematic review research [ 15- 17].
All these tests followed established protocols and are detailed in the methods section in the online appendix (available at http://care.diabetesjournals.org/cgi/content/full/dc10-1135/DC1).
We followed established protocols [14], [18].
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