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It is also not known whether robot-assisted prostate surgery gives better, worse or equivalent long-term cancer control than the traditional methods, either with a four-inch incision or with smaller incisions and a laparoscope.
Current descriptions of infection transfection methods, either with or without the addition of CRISPR/Cas9 targeting, are limited in detail and the extent of optimization.
Both approaches are direct prediction-error methods, either with a directly parametrized observer or with an Extended Kalman Filter as a predictor.
F-actin was visualized by two methods, either with or without cell fixation.
Specifically, the performance of younger adults can be plotted as a function of performance of older adults for all previous investigations (cf., L'Abbé plot [46]) and then find the best fitting theoretical effect size curve (see Figure 4b for examples) using double variate error minimization methods, either with or without weighting each point by its sample size.
When cultures reached approximately 80% confluence in 15-cm culture dishes, medium was replaced with fresh basic MSC culture medium (see above in Multipotent stromal cell isolation and culturing methods) either with 10% FBS or lacking serum.
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The hydrodynamic behaviour of electrochemical reactors with parallel plate electrodes is experimentally studied using the stimulus-response method either with an empty reactor or with different turbulence promoters.
The dataset is constructed from the available Ames test data by using the following criteria: a) standard Ames test data of Salmonella Typhimurium strains required for regulatory evaluation of drug approval; b) Ames test performed with standard plate method or preincubation method, either with or without a metabolic activation mixture.
Sodium intakes were measured differently as mentioned above, either as sodium excretion in 24 h urine, or via a dietary assessment method either with or without questions to determine discretionary sodium consumption.
All blood glucose concentrations will be analysed by the gold standard glucose oxidase method, either with a portable blood glucose analyser (e.g. iSTAT, Abbott Laboratories, Abbott Park, IL USA) or a combined metabolite/blood gas analyser (e.g. ABL 700, Radiometer Ltd ,Copenhagen, Denmark).
Published studies using FISH mostly relied on one of the two methods: either fixation with buffered formaldehyde (BF) with subsequent permeabilization or hypotonic treatment with methanol:acetic acid (75%25%%, MAA) fixation, dropping of nuclei on slides, and air drying (Hypo-MAA).
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