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As shown in Table 4, these three clustering methods displayed a near-perfect ability to detect known subgroups.
Tumor volume (calculated along serial histologic brain sections as described in Materials and Methods) displayed a significant reduction of the tumor mass formed by Daoy cells in GlaB-treated mice compared to the control (Fig 7A and B).
We observed high concordance between the results of the two independent methods (99% of 450 k sites were also represented in the B-SOLANA results) and the methylation levels of cytosines, which were assayed by both methods displayed a very high correlation (Pearson correlation r > 0.93).
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PMOnly methods display a large number of highly correlated gene pairs in the Hasse dataset and in two of the three dual-channel datasets (PramilaS and PramilaL), while LoessOnly methods eliminate a large part of these correlations, especially in the Hasse dataset.
The reciprocal best BLAST hit strategy (RBH), used as first step for most BLAST-based orthology detection methods, displays a low FP error rate (8%), but its inability to recognize many-to-many or many-to-one co-ortholog relationships results in a high FN error rate (30%), as previously expected [4].
These methods display a low-dimensional projection of the data highlighting the main sources of variability.
Both methods display a high specificity of 98%, and can be useful as a rule-in marker.
In all cases, this enhanced Lagrangian method displayed a very good accuracy and computational efficiency and a sharp numerical resolution of shock waves.
The method displayed a wide linearity range and enabled LOD of 0.68 pg/mg, LOQ of 2.4 pg/mg, and precision below 8.12%.
With the final optimized method, the recoveries of the target compounds in Fructus Corni were in the range of 95.2 103% (RSD < 5.0%) and the method displayed a good linearity within the range of 0.8 200 μg mL−1 for morroniside, sweroside, loganin, cornuside and 1.2 300 μg mL−1 for 5-HMF.
The optimized method displayed a quantitative range of 375 4,500 pg/mL.
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