Intracellular metabolite concentrations were determined by anion-exchange chromatography after sample preparation (see Methods, Determination of intracellular metabolite concentrations).
These are grounding methods: determination of the thermal conductivity (hot wire method), determination of microstructure, determination of the mechanical properties, determination of absorption and determination of permeability.
In both methods, determination of percent inhibition of parasitemia is the most reliable parameter.
Genotyping calls were made as described in Methods, (Determination of Genotypes, Real Time OCP ligation/ERCA).
Lignin and carbohydrates were analysed according to the National Renewable Energy Laboratory Laboratory Analytical Procedure NREL LAPP) methods (Determination of Structural Carbohydrates and Lignin) [ 25].
Quality measurements depend on the use of validated methods, determination of the measurement uncertainty, and the availability of reference materials as well as components including appropriate calibrated equipment, trained operators, quality reagents, and the quality of the extracted DNA.
