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These methods detect site-specific signatures of positive selection by estimating the ratio of nonsynonymous/synonymous substitutions (dN/dS) at every codon.
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This method is still conservative in that it averages d N and d S over lineages at each site, but it has improved power to detect site-specific positive selection in a functional protein sequence (Wong et al. 2004).
Then the two datasets were selected to detect site-specific positive selection and purifying selection.
A facile and efficient method for detecting site-specific serine phosphorylation in proteins is therefore highly desirable for both in vitro and in vivo applications.
Using the Datamonkey web server (21, 22 ), we used 3 methods to detect site specific nonneutral selection: single-likelihood counting (SLAC), fixed effects likelihood (FEL), and internal FEL IFELL) (23, 24 ).
Multilead pattern ST-segment "fingerprints" may serve as a noninvasive marker for detecting site-specific reocclusion.
To detect site specific integration at mpsL1 (mice pseudo-site from liver ), a nested PCR approach was followed.
While existing methods detect condition-specific events via binary calls, dCaP detects quantitative variations.
Scintigraphy with labelled autologous WBC is a widely used method for detecting sites of infection.
These indirect methods detect global changes that might not be specific to the channel of interest.
As these methods detect only high occupancies of transcription factors at sites with the strongest affinities, there must be a far greater number of quasi-specific sites with weaker affinities that are similar to the recognition sequences.
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