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Owing to both the amino acid alphabet size and the sheer database size, to become tractable, this step required the development of new computationally efficient methods (detailed later in the text).
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(Image will be detailed later).
Later, the fixed tissues were embedded in paraffin for silver staining according to the Bielschowsky method [30] modified from the Hirano method (detailed description in [31]).
See methods for detailed explanation.
See Methods for detailed description of the ranking analysis.
See Materials and Methods for detailed sample information.
Short descriptions of published methods and detailed descriptions of unpublished methods can be found in the Supplementary Note.
Experimental methods and detailed gene lists.
See methods for detailed character site definitions.
See Materials and Methods for detailed information.
See Supplementary Methods for detailed mapping procedure.
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