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Samples were submitted to the tests/reagents, following the methods described subsequently.
A minimum of four near-adjacent sections were analyzed by each of the methods described subsequently.
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Detailed laboratory methods are described subsequently.
Methods and procedures followed those for simulated data (above), except as described subsequently here.
The detailed procedure is described subsequently.
The two steps are described subsequently.
A quantitative physical activity questionnaire specific to the Indian population was developed using methods described previously, 22 and subsequently validated against reference methods in both rural and urban Indians.
For addition to DC, MVs were isolated according to the method described above, subsequently the MV pellet was resuspended in RPMI medium without FCS, and the protein concentration was determined using the bicinchoninic acid assay (Sigma-Aldrich).
Targeted NGS analysis was performed according to methods described earlier [ 34] and subsequently revised and improved.
Subsequently, 09FD_83 and GC_83 were analyzed using the methods described previously to obtain correlations across experiments.
Subsequently, a pristine FLG flake is transferred over the FeCl3 FLG flake following the methods described in refs (19 and 20).
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