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Although it was possible to simplistically rank the methods based on their relative performance characteristics (Table 2), this was considered an overly simplistic approach, and there was consensus that all the methods described performed very well and yielded valuable results.
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All experimental methods described were performed in accordance with the guidelines of the Canadian Council on Animal Care policy on the care and use of experimental animals, and an ethics protocol approved by the Animal Users subcommittee of the University of Western Ontario Council on Animal Care.
Values are means ± SD The methods described are performed serially on the colon, but the digital acquisition of images and the ability to overlay multiple images permits parallel analysis of colon crypts in a manner not previously reported.
PET/computed tomography (CT) imaging of [18F]BF4 − produced by the optimised high-specific-activity methods described above was performed in 4 8-week-old female BALB/c mice (n = 3 per SA group).
CB implemented the leave-one-out and gradient descent methods described here and performed the run of all the experiments.
Understand that each of the methods described above should be performed at your own risk.
Calcium imaging based on intensity or ratio measurements was performed using methods described previously [28].
All samples were kept frozen, and DNA extractions were performed using methods described elsewhere [34].
These studies were performed using methods described previously [63], with cells grown on 24 well glass bottomed plates, (Iwaki, supplied through Jencons) coated with 0.1% gelatine.
Immunohistochemistry experiments were performed using methods described previously [ 12].
RNAi was performed following methods described previously [ 8, 22].
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