Ag MNPs were synthesized using non-hydrolytic sol gel methods described elsewhere [11, 12, 13].
The chemical composition of the MLP (lignin, hemicelluloses, cellulose, ash and extractive components) was determined following conventional analytical methods described elsewhere (Li et al. 2004).
Overweight patients received a 4-week low-carbohydrate (about 30 g/day carbohydrates), low-fat (fixed 15 g lipids), and normal protein (1.0 1.2 g/Kg of desired weight proteins) VLCKD diet (≤800 kcal) according to methods described elsewhere [8, 9].
We obtained heart rate from the transmitter signals using methods described elsewhere [19].
All samples were kept frozen, and DNA extractions were performed using methods described elsewhere [34].
Primary neonatal rat ventricular cardiomyocytes were isolated from 2-day old neonatal Sprague-Dawley rats using methods described elsewhere [40].
The extracts of E2, P4 and cortisol metabolite in the supernatant were analyzed by enzyme-immunoassay (EIA) according to methods described elsewhere [29].
We used a variety of methods described elsewhere [19], [20] to select a recombination-free 3427-bp fragment from subtype B viruses to analyze the origin of CRF28 and CRF29.
Tissue samples were selected for each case (normal and cancer individuals), and paraffin-embedded 4-µm sections were used and immunostaining was performed according to methods described elsewhere [23], [30].
The ribosomal DNA (rDNA) segment containing the 3'-end of 18S, internal transcribed spacers, 5.8S, and the 5'-end of 28S was amplified, cloned, and sequenced following the methods described elsewhere [15], [26].
Procalcitonin (PCT) assessment followed standard methods described elsewhere [ 12].
