The procedure is based on the through diffusion method classically used for diffusion experiments.
It is coordinate-free and extends the well-known quasi-steady-state method classically used for kinetic scheme reduction.
In chicken, the staining method classically used to distinguish PGCs from the somatic cells was the periodic acid-Schiff (PAS) staining (Fujimoto et al., 1976).
This method is classically used taking into account only geometrical defaults.
Preparation for analysis was minimal and could potentially further robotized, unlike methods that are classically used in lipid analysis or metabolomics.
Therefore, under the assumption that the accumulation of deletion is similar in all families, and occurs continuously through time, we estimated the deletion rate and the deletion loss rate by combining the data obtained for 34 families using a method derived from one classically used in animals ([ 41, 42] and Materials and Methods).
The PAM algorithm is classically used as a supervised method, but we used it as a "mixed unsupervised" method by giving a range of possible numbers of classes instead of a fixed one.
Third, this study did not elucidate advantages of this method over VBM or other sophisticated quantitative methods except by comparing LC changes with IUD, a marker classically used to reflect medial temporal lobe atrophy.
Increasingly more studies are demonstrating that imaging methods, such as computed tomography (CT) and magnetic resonance imaging (MRI), classically used to demonstrate the anatomic position and extent of tumours, are able to provide additional, biological information.
Ternary diffusion coefficients were determined from sorption kinetics with a method of Crank [1975. The Mathematics of Diffusion, second ed. Claredon Press, Oxford] classically used for binary polymer solvent systems.
