By contrast, DNA extracted with the CTAB and Dellaporta methods from certified reference material show a distinctly high molecular weight band.
The accuracy is also evaluated by applying the proposed method to certified reference material (NIST SRM 1643e), for which the result was in a good agreement with the certified values reported for this CRM (95% confidence level).
Method validation was confirmed by the good agreement between the results of the proposed method and certified values of Cd, Cu, and Pb.
Percentage relative standard deviation (% R.S.D). was calculated by using recovered value of each metal from the proposed method and certified value.
The accuracy of the proposed methods was assessed by analyzing certified reference (CRM); Virginia tobacco leaves (CTA-VTL-2).
The accuracy of the analytical methods was confirmed by analysis of certified reference materials of rice flour (NIST 1568a), wheat flour (NIST 1567a) and Tea (NCS DC 73351), with agreement between 82 ± 3% (Cr) and 108 ± 10% (Fe).
Serum creatinine; fasting glucose; fasting insulin; triglycerides; total, HDL, and LDL cholesterol, and high-sensitivity C-reactive protein (hsCRP) were measured in a certified laboratory by standard methods.
Fasting blood glucose, total cholesterol, HDL-c, A1olipoprotein apolipoproteinolipoprotein B100 (ApoB100), LDL-c, and triglycerides were measured by standard laboratory methods in a certified clinical examination laboratory.
Blood and urine cultures will be taken before commencement of antimicrobial therapy and are analyzed by standard microbiological methods at local certified laboratories.
Serum creatinine, urea, cholesterol, triglycerides, CRP, leukocytes, AST, ALT and LDH were measured by standard laboratory methods in a certified laboratory.
