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Current diagnostic methods cannot predict metastatic PCa at a curable stage of the disease.
Network-based methods cannot predict a gene that is not connected to any other node in the network.
Most of these methods cannot predict the mRNA sequence produced from that promoter, and therefore constructing a traditional cDNA microarray to detect their expression is impossible.
PairFold (Andronescu et al., 2005) and RNAcofold (Bernhart et al., 2006) adopt this procedure, but these methods cannot predict general type of interaction such as kissing hairpins.
The first is that given a query protein, SwissPfam and UniProtKB databases are used to extract domain architecture and the corresponding GO terms for each domain; if any GO term for the query protein is missed in these databases, our methods cannot predict this GO term.
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The LNISO method should be used judiciously, keeping in mind that the method cannot predict the absolute stability parameters as accurately as the conventional isothermal stability, and it is most effective for compounds whose degradation pathways follow first order (or pseudo first order) kinetics.
However, this method cannot predict ER or vacuole, but more importantly, their best classifier could only correctly predict the localization of CTL2, whereas the other 15 proteins we investigated experimentally either had no prediction at all or were incorrectly predicted.
Then, the remaining sequences are predicted using SVM-LZ complexity pairwise algorithm, since the sequence alignment method cannot predict all peptide sequences [ 9].
However, for a simple collection of many sequence fragments, the conventional phylogenetic method cannot predict from what phylotypes individual sequences are derived or the phylogenetic novelty of the individual sequences.
Also, because the socio-political and sanitation data are only available as annual updates, our method cannot predict the effects of concentrated sanitation programs such as house-to-house efforts to remove vector breeding containers.
However, unlike protein-coding genes defined by start and stop codons, the ends of miRNA molecules do not have characteristics that can be used to define the mature miRNAs exactly, which made computational miRNA prediction methods often cannot predict the accurate location of the mature miRNA in a precursor with nucleotide-level precision.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com