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However, these methods can fail if the time-varying frequency components cross with each other.
The adjoint method, among other sensitivity analysis methods, can fail in chaotic dynamical systems.
Obtaining biologically accurate alignments for more than two sequences remains a challenge (Notredame, 2007), as even the best methods can fail to readily align conserved motifs (Edgar, 2004).
Many studies have shown that inappropriate inference methods can fail to recover the correct phylogenetic tree from DNA sequences [ 54- 57].
Conventional microbiological methods can fail to isolate agents because of their fastidious in vitro growth requirements or intermicrobial interactions; thus, for agent isolation, we used 2 culture-independent methods (agent-specific PCRs and 16S clone libraries) in addition to conventional bacterial cultures (17, 20 – 20 ).
The strongest form of birth control is abstinence, and temporary birth control methods can fail.
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Thus an H1-conforming Finite Element Method can fail, even with mesh refinement.
The Newton method can fail when computing some complicated forms of governing equations; on the other hand, our method can easily handle this situation.
If the seed points are selected away from the vessel centers and close to pathological regions, the proposed method can fail (wrongly segmenting the pathological region, as well) as shown in Figure 10d.
Thus, while simulation studies are essential to identify conditions under which a method can fail to properly estimate a given parameter, we may not be able to entirely rely upon them to fully validate a method of historical inference.
From this example, we find that our method can fail to identify overlapping peaks as other existing methods do.
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