The partitioning tracer technique is among the DNAPL source-zone characterization methods being evaluated, while surfactant in-situ flushing is receiving attention as an innovative technology for enhanced source-zone cleanup.
In fact, the reference test is assumed to have 100% sensitivity, which can be unrealistic and generate false parameters for the other diagnostic methods being evaluated.
In addition, we also include the modified least-variant set (LVS) [ 15] in the set of methods being evaluated.
The methods being evaluated were also applied to a real loss-of-function RNAi screening dataset to identify genes that modulate paclitaxel sensitivity in breast cancer cells.
The 129 bp product was selected to determine the amount of DNA to add into the BRCA panel, as it was the closest measure to the mean amplicon size of all methods being evaluated (GeneRead V.1: 155 bp (estimated), V.2: 153 bp, Ion AmpliSeq ~197 bp).
The gold standard is ensured to contain no close relatives within the training sets of the methods being evaluated (cutoff=80% identity) and includes 145 proteins from the cytoplasm, 69 from the IM, 38 from the OM, 29 from the periplasm and 18 extracellular.
