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For common organic compounds, empirical methods are quick to compute and have been effectively parameterized.
(Skeptics of Brazelton's methods are quick to note he shilled for Pampers size 6 diapers, i.e. the big ones for toddlers over 35 pounds).
These precipitation methods are quick (~2 h), but non-specific because they are known to also isolate non-exosome biomolecules and cellular debris.
These filter- based methods are quick (<2 h) at isolating exosomes; nevertheless, they have been shown to isolate nano-particle size RNA-protein complexes and chylomicrons whose size (100 350 nm) is similar to the exosomes as well, thus raising concerns about contamination.
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Since the composition-based methods do not involve alignment of query sequences with reference database sequences, these methods are quicker as compared to similarity-based methods.
In theory, the latter methods are quicker, cheaper, and easier to use than flow cytometry and autoradiography and are more reproducible than is mitotic figure counting [ 7].
In theory these methods are quicker, cheaper and easier to use than flow cytometry and autoradiography and more reliable and reproducible than mitotic figure counting.
In addition, second generation methods were quicker, associated with faster recovery times, and associated with fewer adverse procedural events and could be carried out under local anaesthesia.
This recommended method is quick, simple, and secure.
This method is quick but often deceptive, as the leaders' self-reports may be biased.
The T-history method is quick and uncomplicated, however numerous adaptations have been proposed over the years.
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