Sentence examples for methods and transformed from inspiring English sources

To examine the roles of PdeL and PdeH, PDEL and PDEH gene replacement constructs (Figure S1A) were generated (see Materials and Methods) and transformed into the protoplasts of the wild-type strain Guy11.

Bipartite gene deletion fragments were generated as described in Materials and Methods and transformed to AB4.1.

For this, I followed their methods and transformed mutation rate data by taking the log of X + 0.001, while EPC rates were square root – arcsine transformed [ 1].

Two vectors, pMA171-hacA and pMA171-ireA, were constructed as described under " Materials and methods" and transformed into a NC6.2 (ΔhacA) and NC7.1 (ΔireA/ ireA), giving strains NC8 and NC9, respectively.

The image enhancement algorithms can be classified into two categories: spatial domain methods and transform domain methods.

After excluding TFs which were not considered as such by all methods and transforming all gene names to YPD nomenclature, the resulting dataset contained 101 distinct TFs.

Trapezoidal fuzzy membership functions are defuzzified with the weighted average method and transformed into numeric values as weight matrix (Table 2) and initial state vectors.

Trapezoidal fuzzy membership functions are defuzzified with the weighted average method and transformed to [−1, 1] interval as a weight matrix.

For each tweet the words were stemmed using the Snowball method and transformed to lowercase before being split into n grams of size 1-5, retaining 2,000 features, with word frequency normalised for each vector.

Plasmids thus generated were transformed into TOP10 competent bacteria using the heat shock method and transformed bacteria were spread onto LB-Agar plates containing 100 µg/mL ampicillin, and 50 µL of 10 mg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal).

The data were normalized by quantiles, model-fitted using Tukey's Median Polish method and transformed into log2 values.