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MCI-related terms were combined with terms for qualitative methods, and supplemented by manual searches of Google Scholar and the reference lists of included studies.
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SNVs were compared to those found in four different databases (see Methods and Supplement 2).
The reported cross-coupling methodology is orthogonal to many reported conjugation methods and supplements these to enable the introduction of multiple different modifications.
Spheroids maintained in basal media (see 'Materials and methods') supplemented with 1% FBS lost ECAD+ epithelial structures and were mainly comprised of mesenchyme within 20 days of 3D culture.
HUVEC cultures at passages 5 and 9 grown in optimal conditions (see materials and methods) were supplemented with vitamin E (25 μM) for 24 hrs and the percentage of SA- β-gal positive cells was measured.
These results are obtained by numerical approach based on the finite volume method and supplement steady state correlations presented in previous works validated experimentally.
The washed RNA bait-cellulose beads (~1 ml) were incubated with one milligram of protein extract overnight at 4°C in an incubation buffer (see Material and Methods) supplemented with 5 µg tRNA to reduce non-specific binding.
The process of fluorescence recovery after photobleaching was inhibited by pre-incubating cochlear cultures for 20 min in an extracellular medium (EXM, see Materials and Methods) supplemented with 100 μ m carbenoxolone, a broad spectrum inhibitor of connexin channels (36) (Fig. 5B, black traces).
In the CFD simulations, the Reynolds averaged Navier Stokes equations (RANS) for both Eu Eu and Eu La methods were supplemented by applying the k ε, k ω, SST, and BSL-RSM turbulence models for comparison.
Reliable methods for assigning functions to uncharacterized proteins are required to support and supplement these methods.
These variants allow characterization of ligand binding and dynamic properties of the enzyme using NMR and fluorescence methods, supplemented with molecular dynamics (MD) simulations.
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