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Detailed genotyping methods and stringent quality control criteria were described in Zheng et al., [ 25].
Using the same methods and stringent criteria described above, we identified 722 putative FOG-3 mRNA targets.
Urinary samples were then stored at −20°C in the study centers and subsequently sent to the core lab at the Centre Hospitalier Universitaire Vaudois (CHUV, Lausanne, Switzerland) for centralized analyses with standard methods and stringent internal quality controls.
The 22 breast cancer patient samples sequenced with the Agilent SureSelect capture kit were taken from 2776 patients who were genotyped using the Affymetrix SNP 6.0 array in a genome-wide association study; detailed genotyping methods and stringent QC criteria were described in Zheng et al. [ 4].
The 22 breast cancer patients sequenced with the Agilent SureSelect capture kit and the 6 samples sequenced with the Illumina TrueSeq capture kit were part of 2776 patients that were genotyped using the Affymetrix SNP 6.0 array in a genome-wide association study; detailed genotyping methods and stringent QC criteria were described in Zheng et al. [ 13].
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This limitation affects both single-point and collapsing methods and necessitates stringent and exhaustive inspection of cluster plots in association signals of interest.
It also is noteworthy that a previous study, which utilized the most rigorous and stringent methods, was the only study to demonstrate a clinical cure advantage in favor of CI administration of beta-lactams [75].
Thus, based on the findings from BLING I, BLING II was designed with rigorous and stringent methods to answer the ultimate question of whether administration of beta-lactam antibiotics by CI will result in improved outcomes for patients with severe sepsis.
It also is noteworthy that a previous study, which utilized the most rigorous and stringent methods, was the only study to demonstrate a clinical cure advantage in favor of CI administration of beta-lactams [ 75].
The real action of PRP is still under debate, and the scientific community asks for stringent methods and careful evaluations, even for a single case study.
For a direct comparison between the two methods we identified the genes with an adjusted p-value <= 0.05 and absolute fold change >= 2 (Table 1 row 16) for both methods and applied the less stringent proximity filter to the TC 3′ ends.
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