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The commonly used methods for fluoride removal include coagulation, separation, precipitation, adsorption, ion-exchange, electrolysis, electro dialysis, electrochemical methods and reverse osmosis (Meenakshi and Maheshwari 2006; Fawell et al. 2006; Vasudevan et al. 2009, 2011, 2014).
Total RNA was prepared using standard methods and reverse transcription performed with ImpromII™ (Promega, Madison, WI, USA).
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With an emphasis on the different morphologies of TiO2 and the influence factors in the synthesis, this review summarizes fourteen TiO2 preparation methods, such as the sol gel method, solvothermal method, and reverse micelle method.
(a) Chemical structure of nanosized silver (II) pyridoxine complex; (b) absorption spectra of AgNPs and AgPyNPs; (c) TEM image of AgNPs and AgPyNPs synthesized by a modified citrate-reduction method and reverse microemulsion method, respectively; (d) size distribution analysis of synthesized nanoparticles.
RNA was extracted from homogenized heart tissue, using a modified GuSCN-silica method and reverse transcribed using random hexanucleotides (IDT) and 10U of AMV-RT (SBS Genetech) as described previously for another picornavirus [27].
Total RNA was isolated using the TRIzol method and reverse transcribed into cDNA with the PrimeScript RT reagent kit.
On general, for exploration of risk alleles for polygenic conditions, fundamentally two methods have been developed, candidate gene approach method and reverse genetics method including GWAS, one ultimate form of reverse genetics.
Total RNA was extracted from both the phloem-enriched and control tissue using the Trizol method and reverse transcribed using SuperScript II according to the manufacturer's instructions.
The effects of TAE on five CYPs activity and mRNA levels were quantitated by cocktail probe drugs using a rapid chromatography/tandem mass spectrometry (LC-MS/MS) method and reverse transcription-polymerase chain reaction (RT-PCR), respectively.
The perovskite-type SmFeO3 powders were prepared by the four different methods, named decomposition method of heteronuclear cyano complexes (CN), polymer precursor method (PP), reverse micelle method (RM) and reverse homogenous precipitation method (RHP), and their catalytic activities were evaluated with a CO oxidation reaction.
RNA was extracted from 5×1010 cells by the RNAzol method and reversed transcribed by M-MLV-RT and random hexamers as described by the manufacturer.
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