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Using the Volocity software we identified the mRNA granules and the SGs as described in Material and Methods, and quantified their interactions.
DNA was extracted from samples using conventional methods and quantified using PicoGreen (Invitrogen).
M.S. developed the methods and quantified the levels of TMAO, choline, betaine, and bile acids.
DNA was extracted from EDTA-venous blood samples using conventional methods and quantified using PicoGreen (Invitrogen, Carlsbad, CA, USA).
We analyzed the strengths and weaknesses of the methods and quantified the impact of biological and technical challenges.
Total DNA was extracted from three sPD and three CT cybrids by using standard methods and quantified by UV spectrophotometry (λ = 260 nm).
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DNAs were extracted using the CTAB method and quantified using both a NanoDrop ND-1000 Spectrophotometer and agarose gel electrophoresis.
The antibacterial effects of these textiles were evaluated using ISO 20743 standard with the transfer method and quantified by the plate count method.
The ssDNA library of M299 was prepared from five µg DNA according to the GS FLX Titanium Library preparation protocol (Gel-cut method) and quantified and titrated similarly to the standard library by qPCR and test emulsions.
ARV strain S1133 was purified using the CsCl method and quantified as previously described [ 39].
The ribonucleic acid (RNA) was isolated by chloroform-isopropanol method and quantified.
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