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Genomic signature-based identification techniques have the advantage of being precise, highly sensitive, and relatively fast in comparison to biochemical typing methods and protein signatures.
Still, algorithm performance is extremely consistent over both consensus network construction methods and protein family.
In the past, many empirical models of amino acid substitution have been derived using a variety of different methods and protein datasets.
Proteome analyses involved several methods and protein isolation techniques such as ammonium sulphate precipitation, high-performance liquid chromatography (HPLC), SDS-PAGE, and MALDI-TOF/TOF mass spectrometry [ 12].
Soluble proteins and membrane proteins were analyzed separately by two-dimensional (2D) and 1D-PAGE, respectively (see Methods), and protein bands or spots of interest were excised for PMF identification.
Some important objectives in autophagic regulatory mechanism research should be: (1) Investigation of the relationship between JNK and various ATG genes and its impact on autophagy, by means of genetic methods and protein interaction techniques; (2) Investigation of the cross-action between JNK pathway and other autophagy signalling pathways.
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Particular effort was made to investigate different docking methods and protein-ligand binding energy computation methods.
The methods essentially fall into three main categories: arrays, display methods and protein-fragment complementation assays.
Proximate composition of samples was carried out using the AOAC Official Methods of Analysis [ 16], with analysis of moisture (method 925.04), ash (method 938.08), fat (method 954.02), crude fibre (method 985.29) and protein (method 981.10).
Proximate analyses including moisture (gravimetric method), ash (gravimetric method), fat (Soxhlet extraction method), and protein (Kjeldahl method) contents in accordance with the standard methods (Association of Official Analytical Chemist AOAC 2006), while carbohydrate contents were analyzed using the phenol-H2SO4 method (Dubois et al. 1956).
His-TRAIL was quantified by the Bradford method and protein assay reagent (BioRad, Richmond, CA, USA).
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