Sentence examples for methods and primer from inspiring English sources

Many human factors probably contributed to the species sampling variability including motivation, funding and improvements in molecular methods and primer design.

Using different extraction methods and primer pairs [62] a region of 654 bp was sequenced across the origin (from position 15,759 to 73), covering the tRNA-Pro gene and 68% of the 5'-end of the control region.

These methods and primer sequences have been described elsewhere (26) and are included in full in Supplement 1.

messenger RNA quantification was performed by real-time PCR using custom made human and rabbit quantification multi-gene plasmid standards based upon methods and primer pairs as previously described (Corware et al, 2011; Schnupf & Sansonetti, 2012; Shaunak et al, 2004).

Cultured INS-1 832/13 cells or isolated rat islets and human islets were washed with PBS and quantitative RT-PCR was performed using previously published methods and primer sequences.

Different methods and primers for RNA extraction from plasma, cDNA synthesis, nested PCR and sequencing on an ABI310 automated sequencer were evaluated and optimised.

HIV gag, pol and nef were sequenced using previously described methods and primers [17].

DNA sequencing was performed using methods and primers as previously described.

Semi-quantitative RT-PCR and real-time PCR were performed using methods and primers described in Text S1 and Table S1.

NS32 of SMReV was also cloned into pcDNA3.1 using the same methods and primers NS32-F/S7-R.

In addition, European bison and wild boar GHR exon 10 were analysed using same methods and primers as described in [ 10].