Sentence examples for methods and prepared from inspiring English sources

We have now modified this procedure (see Materials and Methods) and prepared genomic DNA by embedding cells in agarose plugs followed by spheroplasting.

To confirm expression of hPINK1 in the transduced fibroblast cell lines, total RNA from these cell lines were extracted using standard methods and prepared for subsequent analysis.

Cochrane Collaboration review groups, including the Cochrane Musculoskeletal, Cochrane Menstrual Disorders and Subfertility, and the Cochrane HIV/AIDS Groups, have developed methods and prepared overviews to address these types of questions [ 14- 16].

HCY conceived of the study, developed statistical methods and prepared the manuscript.

HCY conceived the study, developed the statistical methods, and prepared the manuscript and software user guide.

PG carried out statistical analyses including life-table methods and prepared the first draft.

More recently, different groups have used chemical vapor deposition method and prepared aligned carbon nanotubes (ACNTs) [17 23].

We applied this concept to our new foam contrast using the simple and easy Tessari's method and prepared human serum albumin acting as the protein shell and room air as encapsulated gas.

We extracted total RNA from each NSCLC cell line by using Trizol method and prepared cDNA using SuperScript-II RT Kit (Invitrogen) and Oligo (dT s according to the manufacturer's instructions.

The tissues were then washed with 1xTBST and incubated for 30 minutes at room temperature with biotinylated secondary antibody solution from the Dako REAL EnVision Detection System (Dako, Denmark), washed with distilled water, counterstained with hematoxylin (SIGMA-ALDRICH, Inc., USA), dehydrated and clarified by a conventional method, and prepared for examination under a light microscope.

AJ – Helped create the database, performed statistical analysis, interpreted results, drafted the methods section and prepared figures, revised the initial draft of manuscript, and approved the final version of the manuscript.