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Taken together, we describe methods and precautions to reliably detect and inactivate LPS in polysaccharide preparations to allow reliable in vitro investigations towards immune-modulatory potential of polysaccharide preparations.
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Essential techniques of our surgical method and precautions for avoiding risks are summarized as follows: Arthroscopic portals should be established correctly to provide an easy path to the coracoid process and proper working space.
Further details of the method, and some precautions, are noted in Altman and De Stavola (1994).
Persons who dispatch sick wildlife are encouraged to use appropriate, humane methods and should take precautions against exposure to tissues and body fluids.
Of these 21 genes, 15 showed evidence of mitochondrial-characteristic RNA editing (Table 1; see Methods for all controls and precautions taken to ensure that the inferred RNA edits are all real).
Based on the results obtained using these methods at laboratory scale, recommendations and precautions for processing at larger scale are made.
The methods and materials used and the precautions taken regarding pre-PCR work, chemicals, reagents etc. are described in detail in Melchior et al. (2008) [15], [16].
The procedures and precautions for the application of trenchless methods on narrow pavements are proposed.
On the basis of observations, a set of criteria and precautions are suggested for adoption with both PS and CS methods in the aquifer characterization of highly urbanized areas.
The molecular methods used in our study have been previously validated (7, 8 ), and precautions were taken to reduce risks for contamination during processing.
The meticulous examination of putative contaminations and precautions we have undertaken are now stressed more in details in the revised manuscript (Methods section).
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