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Differentiation of KO and reconstituted cells into brown adipocytes was performed as described in Methods and outlined in Fig. 1B.
We have provided a summary of the advantages to and bottlenecks in existing methods and outlined some open questions.
Assays performed in white solid-bottom 1536-well plates used the protocols described in Supplementary Methods and outlined in Supplementary Table 1.
We obtain a homogeneous RNA sample from the parental EST clone vector as described in Methods and outlined in Figure 1.
The functional annotation grouped genes into a high confidence set and a low confidence set based on the criteria described in "Methods" and outlined in Additional file 4 Figure S3.
To identify new protein-coding genes that were missed in previous genome annotations [ 20, 33], we followed the procedure described in Methods and outlined in Additional file 1: Figure S2.
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In this review, we introduce these methods and outline a procedure for applying this analysis directly to experimental data.
In Part II, we review various physical/chemical methods and outline the principle, experimental implementation and data processing of each technique.
In this review, we provide an overview of protein peptide docking methods and outline their capabilities, limitations, and applications in structure-based drug design.
We summarize the advantages of and bottlenecks in the existing methods and outline some open problems in the field.
The "Measurement and data" section describes the estimation method and outlines our data sources.
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