Sentence examples for methods and normalized from inspiring English sources
To examine this, we profiled temporal cortex mRNA levels by qRT-PCR for 4 of the "type 1" genes listed in Fig. 2 (SYK, NEUROD2, GABRA2, GAD1) in a cohort of six child brains (range: 1.3 11.5 years) and 11 adults (range: 32 87 years), carefully matched for RNA quality (see Methods) and normalized to 18S rRNA levels (data not shown).
Soluble scFvs were produced in HB2151 bacterial strain, as described in Materials and Methods, and normalized by ELISA on anti-polyhistidine antibody-coated plates.
For all pairs of methods from the Supervised Machine Learning Top-5, we counted the number of abstracts that mention both methods and normalized the counts with the number of co-occurrences that would be expected by chance (based on the frequencies with which the methods are mentioned over the years).
Reverse transcription PCR primers were designed between different exons to avoid any amplification of contaminating DNA (Additional file 1: Table S8) and the cDNA levels of target genes were analyzed using comparative Ct methods, and normalized to the level of β-actin.
The pixel variation between each frame was quantified for each video to give a continuous signal relating to average motion of the mouse (motion index calculated as described in the 'Materials and methods' and normalized to the maximum value in the video).
After scanning, background correction was done to reduce the effect of background noise, followed by normalization with the Lowess method, and normalized array data were fitted into linear models to detect differential expression over different stages.
Expression levels were normalized to the average of the housekeeping genes using the delta Ct method, and normalized expression is calculated as 2^-deltaCt.
The average fitness factor method and normalized weighted objective functions method are separately used to select the best optimal solution from Pareto optimal fronts.
mRNA levels were calculated with an absolute quantification method and normalized by the amount of G3PDH for each sample.
Relative transcript level for each gene was determined by using 2−ΔΔCt method and normalized to housekeeping RPL19 transcript level.
Amount of DNA immunoprecipitated with FLAG and IgG beads was calculated based on threshold cycle [C t)] using the ΔC t) method and normalized to input samples.
