The DBN learning models are developed in two stages: (1) the initial models are developed using unsupervised learning methods with unlabeled data streams and (2) the models are fine-tuned using supervised learning methods and labeled data streams.
BAC DNAs were extracted by conventional methods and labeled with green or red/orange fluorophores using a non-enzymatic nucleic acid labeling method (ULS™, Kreatech Diagnostics, Amsterdam, The Netherlands).
Briefly, test (cell lines) and reference (peripheral blood lymphocytes from healthy donors) DNA was extracted using standard methods and labeled in nick translation reactions using SpectrumGreen- and SpectrumRed-conjugated nucleotides (Vysis, Downers Grove, IL).
In Muslim countries, meat is slaughtered using the halal method and labeled as such.
Total RNA was reverse-transcribed to cDNA, amplified by a modified Round A/B random PCR method, and labeled with Cy3 or Cy5 fluorescent dye as previously described [7], [8].
A small number of cores were damaged during TMA construction or subsequent methods and were labelled as noninformative.
BACs RP11-806M4 and RP11-42N21 wextractedcted using standard methods and then labelled by nick translation in spectrum orange (Abbott, Rungis, France) and in spectrum green (Abbott), respectively.
Specimens were cleared by centrifugation according to standard methods, and were labelled with a study number such that all analysis was blinded.
Wild-type and mutant clones were induced using the Mosaic Analysis with a Repressive Marker (MARCM) method [32], and labeled with UAS-mCD8 GFP. UAS-mCD8 GFP
