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The DBN learning models are developed in two stages: (1) the initial models are developed using unsupervised learning methods with unlabeled data streams and (2) the models are fine-tuned using supervised learning methods and labeled data streams.
Briefly, test (cell lines) and reference (peripheral blood lymphocytes from healthy donors) DNA was extracted using standard methods and labeled in nick translation reactions using SpectrumGreen- and SpectrumRed-conjugated nucleotides (Vysis, Downers Grove, IL).
BAC DNAs were extracted by conventional methods and labeled with green or red/orange fluorophores using a non-enzymatic nucleic acid labeling method (ULS™, Kreatech Diagnostics, Amsterdam, The Netherlands).
On the basis of the deviance information criterion, we inferred the most likely number of populations to be nine (Materials and Methods) and labeled each population by the most common geographic location and/or substrate from which the strains were originally isolated (Table S2).
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The bread is made using artisan methods and labelled for different blood groups.
Briefly, test (tumour) and reference (peripheral blood lymphocytes from a healthy female) DNA was extracted using standard methods and labelled in nick-translation reactions using fluorochrome-conjugated nucleotides, after which DNA fragment lengths between 300 and 2000 bp were obtained.
In Muslim countries, meat is slaughtered using the halal method and labeled as such.
Total RNA was reverse-transcribed to cDNA, amplified by a modified Round A/B random PCR method, and labeled with Cy3 or Cy5 fluorescent dye as previously described [7], [8].
Wild-type and mutant clones were induced using the Mosaic Analysis with a Repressive Marker (MARCM) method [32], and labeled with UAS-mCD8 GFP. UAS-mCD8 GFP
Good agreement between the results utilizing the three methods and the labeled values was achieved, corroborating the good performance of the proposed electrochemical methodology for ambroxol analysis.
Basically, the solutions to cosegmentation can be roughly classified into two categories: clustering based methods [5 7, 19] and labeling based methods [3, 8 11, 18].
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