Sentence examples for methods and grown from inspiring English sources

50 µl of distilled water was then added to each reaction and competent JM109 E.coli cells (Promega: #L2001) were chemically transformed with 10 µl of the diluted ligation reaction as per standard methods and grown overnight at 37°C on LB (Luria broth) agar media supplemented with ampicillin using standard (X-Gal/IPTG) blue/white selection.

Many of these concerns also apply to crop varieties developed using conventional breeding methods and grown under traditional farming practices (ICSU).

Extended Experimental Procedures Strains and Media S. cerevisiae strains with genomically encoded tagged proteins were generated by standard methods and grown at 30°C to A600 ∼ 0.5.

S. cerevisiae strains with genomically encoded tagged proteins were generated by standard methods and grown at 30°C to A600 ∼ 0.5.

The cells were plated into cell culture inserts as described (see Materials and Methods) and grown up to four days after confluence, when they had already formed a 3 4 layered epithelium, and TER values were determined.

Five independent transposon libraries were constructed in S. Typhimurium ATCC 14028 (STM1) and two in S. Typhi Ty2 (STY1), using the EZ-Tn5 < KAN-2 > Promoter Insertion Kit (Epicentre Biotechnologies) (see methods), and grown in Luria broth (Additional file 1: Table S1).

This number will surely increase due to improved contact prediction methods and growing sequence databases at increasing rates.

Uniform ZnO NRs were obtained by hydrothermal method and grown on a graphene surface that had been transferred to a PET substrate.

The uniform ZnO NRs were obtained by hydrothermal method and grown on a graphene surface that had been transferred to a polyethylene terephthalate substrate.

Diamond was nucleated on mirror-polished silicon substrate by the bias-enhanced nucleation (BEN) method and grown by microwave-plasma chemical vapor deposition (MWPCVD) technology.

Both cultures were established using the same method and grown for approximately the same number of population doublings.