306 204 102 0.10 0.37 S BET, Sμ and S Ext are, respectively, surface area determined by the BET method, microporous surface determined by the t-plot method and external surface determined by difference, and are given in m2g−1.
Fasting glucose was determined at a central laboratory using glucose-peroxidase method and external quality control.
For verification of the iTRAQ method and external validation in a larger cohort, two proteins were selected for immunoblot validation.
Serum cotinine was quantified using high-performance liquid chromatography/tandem MS using an isotope dilution method and external standard calibration plots (Bernert et al. 2009).
Two proteins, serum amyloid A-4 (Sand) and astacin-like metalloendopeptidase (ASTL), were selected for verification of the iTRAQ metalloendopeptidasevASTLation were immunoblot in a larger cohort with mixed hiselected in plasma (n = 68), and cyst forid (n = 68).
In this method, internal and external perspectives are evaluated according to the overall situation of the company in the market to build strategies basing on the factors in the four main groups (FS, CA, ES, and IS).
The built model was fully assessed by various validation methods, including internal and external validation, Y-randomization test, chemical applicability domain, and all the validations indicate that the QSAR model we proposed is robust and satisfactory.
Methods: Bladder pressure and external urethral sphincter electromyogram (EUS EMG) were recorded in α-chloralose/urethane anaesthetised guinea-pigs during rapid infusion of cold or warm saline into the bladder with or without prior intravesical exposure to menthol or resiniferatoxin (RTX).
There are two common validation methods— cross-validation and external validation (Tong et al. 2003c).
