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These results suggest that current neuralization conditions (see methods and expression analysis below) favor the derivation of dorsal neuroepithelium, which is the region of the neural tube from which NC arises in vivo [36], [37].
Human embryonic kidney (HEK293) cells were transiently transfected with ADAMTS13 plasmid DNA as described in the Materials and methods and expression of ADAMTS13 in this system has previously been shown to be equivalent to that in human stellate liver cells, which are known to express ADAMTS13 at high levels [25].
Subsequently, data normalization was performed using RMA methods, and expression differentiation analysis was conducted using limma methods.
Other studies have identified genes upregulated in or specific to zebrafish gonads, based on various methods and expression criteria [ 28, 42, 43].
Reaction conditions were as described in Materials and methods, and expression was assessed relative to that of S28, the internal reference gene.
Several approaches have been developed to predict or to discover imprinted genes in various tissues including computational methods and expression profiling of tissues carrying uniparental disomies (Schulz et al., 2006; Luedi et al., 2007).
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Late medieval church music became progressively more direct in method and expression.
Also, an improved sensor design, data processing method and expression of test results for the fabric moisture management properties are introduced.
We compare GO enrichment between the co-training method and expression similarity alone.
Quantitative measurements were determined using the ΔΔCt method and expression of GAPDH was used as the internal control.
Quantitative measurements were determined using the ΔΔCt method and expression of β-actin was used as the internal control.
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methods and interpretation
methods and language
methods and demonstration
means and expression
methods and voices
approach and expression
methods and use
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methods and assessment
methods and instrumentation
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methods and climate
method and expression
methods and expressed
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