Sentence examples for methods and expressed from inspiring English sources

He said he opposed coercive methods and expressed objections privately to colleagues.

The dielectric permittivity of susceptible and resistant strains of Escherichia coli and Staphylococcus aureus, treated with gentamicin and vancomycin, were measured directly using differential impedance sensing methods and expressed as the Normalized Impedance Response (NIR).

Considering the G-rich nature of the sequence, PG4 density was normalized for GC content (which was noted to be significantly different in hotspots versus coldspots (Methods)) and expressed as RPG4/GC (Methods).

We next performed a protein structure analysis [21] [23], by introducing a moving average, S, of local hydrophobic scores around each AA site (Materials and Methods), and expressed the local hydrophobic environment of the root sequences using an AA scale model (Table S1 gives the hydrophobic score of each AA with an orthodox model [24]).

We measured the concentrations of Leu and Ile in the culture media using a bioassay (see Methods), and expressed these as the cell concentration of L– and I– cells which can be produced by the amount of amino acid supplied, respectively.

Median PFS and OS were estimated using Kaplan Meier methods and expressed with 95% CI.

Results were calculated using the ΔΔCt method and expressed as a percentage of expression compared with SH-SY5Y cells±S.D., with EFNB2 mRNA in SH-SY5Y cells assigned an expression level of 100%.

The mRNA expression was determined using the Livak method and expressed as fold changes [ 31].

Gene expression levels normalized to endogenous control gene expression were analyzed using the ΔΔCt method and expressed as mean percentages ± SEM relative to the WT condition.

Mean (±SEM) fold change in Glast expression for Clock/Clock astrocytes was calculated using the ΔΔCt (deltadeltaCt) method and expressed as a function of wild-type values.

Relative mRNA expression levels were quantified using the comparative Ct(Δ Ct) method and expressed as 2− ΔΔ C t).