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After the first step, the model enzyme was optimized by computational methods and directed evolution.
At the same time, protein engineering by mutagenic methods and directed evolution has been employed to tailor these enzymes.
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He wrote the screenplays for Atonement and A Dangerous Method, and directed Carrington, has worked on operas with Philip Glass and co-wrote the lyrics for Sunset Boulevard with Andrew Lloyd Webber.
JS developed the DXA imaging method and directed the analysis of the breast scans.
Eigen divided the methods used to disturb systems into indirect, or competition, methods and direct, or perturbation, methods.
We use Markov chain Monte Carlo methods and direct numerical integration for inference.
Biomass conversion processes may be divided to the thermochemical, chemical, biochemical methods and direct combustion.
Gutierrez and Chopra (1978) evaluated the methods for seismic analysis of SSI, namely, simple general substructure methods and direct finite element method.
This was unavoidable as low-grade activity in many of the tumours meant that automated methods and direct ROI placement on the PET images proved impossible.
Different methods and methodological approaches suitable to a valuation of recreation based on various principles and criteria (preference and non-preference methods, cost-based methods, revenue-based methods, and direct and indirect methods) are analyzed.
From those models, estimated transpiration ranged from 55% (CFSTR) to 74% (PFR) of total evapotranspiration (ET) rates, consistent with published values using standard methods and direct measurements.
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