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However, many studies analyzed different staining patterns, methods, and cutoff values to define EMH positivity.
The method is relatively insensitive to data normalization methods and cutoff selection and uses rigorous statistical analysis.
Besides AJCC staging, several studies have suggested various tumor sizing methods and cutoff points with prognostic powers in osteosarcoma [ 2- 8].
We have no data on the methods and cutoff points for receptor status used by each laboratory because we obtained this information from the CSP, which bases its classifications on information in pathology reports from a variety of laboratories.
Although many methods and cutoff values have been suggested, most studies have reported on the prognostication of their sizing method using a cutoff value optimized in their study populations.
Muscle function may be determined by performance (eg, gait speed) and/or strength (eg, handgrip strength) measures, 1, 33, 34 but also in this case, the diversity of methods and cutoff values employed impedes study comparability.
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This might be justified by differences in the applied methods and cutoffs to assess posttraumatic stress.
Methods and cutoffs for local assessment of ER, PR, and HER2 varied during the years encompassed by this study.
Treating them on an equal footing goes most of the way: this means procuring from the authors the raw data, analyzing all studies with exactly the same statistical methods and cutoffs, and working only on the universe of common genes.
Efforts are ongoing to improve the performance of IHC-based biomarker testing by standardizing quality control, training, methods and cutoffs, particularly with ER [ 36], Her2 [ 37] and Ki-67 [ 38].
Possible explanations for our lack of correlation between CCND1 amplification and LNM are the differences in the used detection method and cutoff values for amplification between these studies.
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