Sentence examples for methods and copies from inspiring English sources

Further details on the assessment methods and copies of the research instruments are available online at http://www.jhsph.edu/dept/ih/IIP/projects/catalyticinitiative.html.html

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We determined the gene copy numbers for 576 healthy individuals from five different populations using these three methods, compared the copy number distributions generated by the different methods, and compared the copy number distributions across the different populations.

To investigate whether altered K-Ras activity could additionally result from gene amplification, a K-Ras gene copy number assay was designed and carried out as described in Materials and Methods and K-Ras copy number compared in normal and tumour tissues.

In order to identify common distinct CNV regions increasing the risk of RM, nine prioritized CNVRs were selected based on the genome-wide CNV screening results (Supp. Table S3; Supp. Materials and Methods) and subjected to copy number typing using TaqMan qPCR in the discovery sample set (n = 70) (see also Fig. 1).

CA, USA), as described by Providenti et al [39]: ΔCt was determined using the y-axis intercept method and the copy number calculated using FΔCt, where F = 2 if the efficiency is 100%.

Quantitative RT-PCR analysis was performed with Lightcycler 480 software, the threshold cycle was automatically calculated by the second-derivative maximum method, and the copy number of the specific mRNA in the experimental samples was calculated by extrapolation from the gene-specific standard curve.

This data set was specially designed for the development and evaluation of low-level analysis methods for genotyping and copy number estimation from probe intensity data (see, e.g., [22]).

Oncoscan copy number data were processed and normalized by Affymetrix according to previously published methods [ 21], and copy number is calculated in reference to Oncoscan data from an Affymetrix panel of normal reference samples.

> -wrap-foot> We reconstructed a phylogenetic tree using 354 single-copy proteins shared among selected organisms (fig. 2) (see Materials and Methods: Single-Copy Core Genes and Phylogeny).