Sentence examples for methods and cells from inspiring English sources

Intranuclear BrdU staining was done using a BrdU flow kit as described in the methods, and cells were analyzed by flow cytometry.

Cell viability was tested by Trypan blue dye exclusion methods, and cells with <90%% viability were discarded.

Developments in tissue-engineering methods and cell-sheet technology have improved scaffold materials for oesophageal repair.

This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages.

Different in vitro test methods and cell lines have been used to determine dental materials' cytotoxicity.

Cell viability was analyzed by Trypan blue dye exclusion (Supplementary Materials and Methods) and cell cycle distribution was assessed by FACS analysis as described previously.

Detailed description of cell culture, immunoprecipitation, limited proteolysis, Ab-1 staining, transfection methods, and cell death analysis are provided in the Extended Experimental Procedures.

Possible techniques to isolate MSCs from BM materials include cell adherence-based methods and cell-sorting methods, with the vast majority of previous clinical trials using the former method.

Therefore, it is clear that independent groups, using different methods and cell lines, have shown ORC and CDC6 to be at least transiently attached to the NM.

In this study, we used neurosphere methods and cell sorting with CD133 as a marker in order to investigate the biology of GSCs.

This indicated the presence of a statistically significant overlap in genes affected despite the different LSD1 inhibition methods and cell lines used.