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The tools, which comprise both computational methods and cell culture platforms, will measure toxicology from a network perspective, by examining varieties of interactions and processes within the cell.
This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages.
Different in vitro test methods and cell lines have been used to determine dental materials' cytotoxicity.
Therefore, it is clear that independent groups, using different methods and cell lines, have shown ORC and CDC6 to be at least transiently attached to the NM.
Detailed description of cell culture, immunoprecipitation, limited proteolysis, Ab-1 staining, transfection methods, and cell death analysis are provided in the Extended Experimental Procedures.
Cell viability was analyzed by Trypan blue dye exclusion (Supplementary Materials and Methods) and cell cycle distribution was assessed by FACS analysis as described previously.
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We use structural, biochemical methods and cell-based assays to understand how cPCDHs form supramolecular inter-cellular signaling assemblies.
Developments in tissue-engineering methods and cell-sheet technology have improved scaffold materials for oesophageal repair.
Intranuclear BrdU staining was done using a BrdU flow kit as described in the methods, and cells were analyzed by flow cytometry.
Possible techniques to isolate MSCs from BM materials include cell adherence-based methods and cell-sorting methods, with the vast majority of previous clinical trials using the former method.
Cell viability was tested by Trypan blue dye exclusion methods, and cells with <90%% viability were discarded.
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