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The sample data collected from assays shown in Figure 4B summarizes and compares Ca2+ accumulation and P i release in microsomes for three separate conditions: control (no additions to standard assay, see materials and methods), and addition of either 100 nM TG or 10 μM A23187.
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Further information was sought on consumption frequency for different sub-items, preparation methods, and additions.
For the same purpose, two mixing methods and the addition of recycled tyres steel fibres (RTSFs) are also investigated.
Our results are robust to the battery of robustness checks described in Section 5 that include the use of different control groups, different reweighting methods, and the addition of group-specific time fixed effects.
We used different methods and in addition observer triangulation to strengthen validity and relevance as well as credibility, confirmability and transferability in the study [ 24].
With these data, we demonstrate that C3NET gives better results than all other inference methods and in addition has a computational complexity that is among the lowest.
The target record shows two putative amplicons for each of the selected methods and, in addition, several other ones from element-specific methods, all of them confirmed by the JRC GMO-Matrix decision support tool.
We present the frequency of these methods and in addition cited some articles that give a good example of how these selection techniques can be described in the methods section.
The tested chemical transfection methods and in addition the conventional electroporation were not suitable for efficient transfection either due to low transfection efficiencies, formation of vacuoles and lipids or high toxicity.
We described further molecular detection of PPRV from the paper matrix by direct amplification methods and in addition, using Bayesian analysis, we studied the evolutionary relationship of the viral strains involved in outbreaks that occurred in the Southern Asian region.
By using three different methods, and in addition repeating some analyses for subsets of the data, the risk of type I error, i.e. incorrectly discarding H0 of no clustering, increased.
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